Journal: Oncology Reports

Article Title: Pyrogallol-induced endothelial cell death is related to GSH depletion rather than ROS level changes

doi: 10.3892/or_00000635

Figure Lengend Snippet: Figure 1. Effects of PG on cell growth and ROS levels in ECs. Exponentially- growing cells were treated with the indicated concentrations of PG for 24 h. (A and B) Cell growths were assessed by an MTT assay. (C and D) ROS levels in ECs were measured using a FACStar flow cytometer. Graphs indicate DCF (ROS) levels (%) in CPAEC and HUVEC compared with each control group cell, respectively. (E and F) Graphs indicate DHE (O2 .-) levels (%) in CPAEC and HUVEC compared with each control group cell, respectively. *P<0.05 compared with the PG-untreated control cell group.

Article Snippet: Calf normal pulmonary artery endothelial cells (CPAEC) from KCLB (Korean Cell Line Bank) and primary human umbilical vein endothelial cells (HUVEC) from PromoCell GmbH (Heidelberg, Germany) were maintained in humidified incubator containing 5% CO2 at 37 ̊C.

Techniques: MTT Assay, Flow Cytometry, Control

Journal: Oncology Reports

Article Title: Pyrogallol-induced endothelial cell death is related to GSH depletion rather than ROS level changes

doi: 10.3892/or_00000635

Figure Lengend Snippet: Figure 2. Effects of PG on GSH levels in ECs. Exponentially-growing cells were treated with the indicated concentrations of PG for 24 h. GSH levels in ECs were measured using a FACStar flow cytometer. (A and B) Graphs show the percent of (-) CMF (GSH depleted) cells in CPAEC and HUVEC, respectively. (C and D) Graphs indicate mean CMF (GSH) levels (%) in CPAEC and HUVEC except (-) CMF (GSH depleted) cells compared with each control group cell, respectively. *P<0.05 compared with the PG-untreated control cell group.

Article Snippet: Calf normal pulmonary artery endothelial cells (CPAEC) from KCLB (Korean Cell Line Bank) and primary human umbilical vein endothelial cells (HUVEC) from PromoCell GmbH (Heidelberg, Germany) were maintained in humidified incubator containing 5% CO2 at 37 ̊C.

Techniques: Flow Cytometry, Control

Journal: Oncology Reports

Article Title: Pyrogallol-induced endothelial cell death is related to GSH depletion rather than ROS level changes

doi: 10.3892/or_00000635

Figure Lengend Snippet: Figure 3. Effects of NAC and BSO on the cell growth, cell death and MMP (Δæm) in PG-treated CPAEC. Exponentially-growing CPAEC were treated with PG for 24 h following 30 min pre-incubation of 2 mM NAC or 10 μM BSO. (A) Graph shows the cell growth, as assessed by an MTT assay. (B) Graph shows the percents of annexin V-FITC staining cells. (C) The graph shows the percents of Rhodamine 123 negative [MMP (Δæm) loss] cells, as measured with a FACStar flow cytometer. *P<0.05 compared with the control group. #P<0.05 compared with cells treated with PG only.

Article Snippet: Calf normal pulmonary artery endothelial cells (CPAEC) from KCLB (Korean Cell Line Bank) and primary human umbilical vein endothelial cells (HUVEC) from PromoCell GmbH (Heidelberg, Germany) were maintained in humidified incubator containing 5% CO2 at 37 ̊C.

Techniques: Incubation, MTT Assay, Staining, Flow Cytometry, Control

Journal: Oncology Reports

Article Title: Pyrogallol-induced endothelial cell death is related to GSH depletion rather than ROS level changes

doi: 10.3892/or_00000635

Figure Lengend Snippet: Figure 4. Effects of NAC and BSO on ROS and GSH levels in PG-treated CPAEC. Exponentially-growing CPAEC were treated with PG for 24 h following 30 min pre-incubation of 2 mM NAC or 10 μM BSO. ROS and GSH levels in CPAEC were measured using a FACStar flow cytometer. (A and B) Graphs indicate DCF (ROS) and DHE (O2 •-) levels (%) compared with control CPAEC, respectively. (C and D) Graphs show the percents of (-) CMF (GSH depleted) cells (C) and mean CMF (GSH) levels compared with control CPAEC (D). *P<0.05 compared with the control group. #P<0.05 compared with cells treated with PG only.

Article Snippet: Calf normal pulmonary artery endothelial cells (CPAEC) from KCLB (Korean Cell Line Bank) and primary human umbilical vein endothelial cells (HUVEC) from PromoCell GmbH (Heidelberg, Germany) were maintained in humidified incubator containing 5% CO2 at 37 ̊C.

Techniques: Incubation, Flow Cytometry, Control

Journal: Polymers

Article Title: Role of Curing Temperature of Poly(Glycerol Sebacate) Substrates on Protein-Cell Interaction and Early Cell Adhesion

doi: 10.3390/polym13030382

Figure Lengend Snippet: Human umbilical endothelial cells (HUVECs) biocompatibility on PGS substrates. HUVECs viability results from AlamarBlue TM colorimetry technique. Glass was used as a control of good cell viability, and a 10% DMSO solution was used as cytotoxic control. Sample data distributions were analysed through a two-way ANOVA and Bonferroni post hoc multiple mean comparison test with no significant differences between samples.

Article Snippet: Endothelial cells (HUVECs) were purchased from Cellworks, Buckingham, United Kingdom, and supplemented endothelial cell medium was provided by Innoprot, Bizkaia, Spain.

Techniques: Colorimetric Assay, Control, Comparison

Journal: Polymers

Article Title: Role of Curing Temperature of Poly(Glycerol Sebacate) Substrates on Protein-Cell Interaction and Early Cell Adhesion

doi: 10.3390/polym13030382

Figure Lengend Snippet: HUVECs FA quantification on PGS substrates. ( a ) Immunofluorescence images of HUVEC nuclei (cyan), actin cytoskeleton (green) and vinculin (magenta) as a focal adhesion (FA) marker of cells cultured after 3 h on glass and different PGS cured materials. Quantification of ( b ) the amount, ( c ) area and ( d ) average size of FA. FA area was calculated as the percentage of the total cell area. All immunofluorescence images share the same scale bar (20 µm). Sample data distributions were analysed through a one-way ANOVA and Bonferroni post hoc multiple mean comparison test, with a p -value < 0.05. *, p < 0.05 and ** p < 0.01.

Article Snippet: Endothelial cells (HUVECs) were purchased from Cellworks, Buckingham, United Kingdom, and supplemented endothelial cell medium was provided by Innoprot, Bizkaia, Spain.

Techniques: Immunofluorescence, Marker, Cell Culture, Comparison

Journal: Polymers

Article Title: Role of Curing Temperature of Poly(Glycerol Sebacate) Substrates on Protein-Cell Interaction and Early Cell Adhesion

doi: 10.3390/polym13030382

Figure Lengend Snippet: FA parameters of HUVECs cultured on PGS substrates pre-treated in ( a – c ) sequential and ( d ‒ f ) competitive protein adsorption experiments. HUVECs fluorescence quantification of ( a , d ) number of FA, ( b , e ) relative surface of FA compared to the total cell area and ( c , f ) average size of FA, from protein sequential and competitive adsorption assays on glass and PGS cured at different temperatures. FA area was calculated as a percentage (%) of the total cell area. Sample data distributions were analysed through a two-way ANOVA and Bonferroni post hoc multiple mean comparison test, with a p -value < 0.05. *, p < 0.05; **, p < 0.01; ***, p < 0.001; and ****, p < 0.0001.

Article Snippet: Endothelial cells (HUVECs) were purchased from Cellworks, Buckingham, United Kingdom, and supplemented endothelial cell medium was provided by Innoprot, Bizkaia, Spain.

Techniques: Cell Culture, Adsorption, Fluorescence, Comparison